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An easy method for preserving nucleic acids in field samples for later molecular and genetic studies without refrigerating
Author(s) -
Laulier M.,
Pradier E.,
Bigot Y.,
Périquet G.
Publication year - 1995
Publication title -
journal of evolutionary biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.289
H-Index - 128
eISSN - 1420-9101
pISSN - 1010-061X
DOI - 10.1046/j.1420-9101.1995.8050657.x
Subject(s) - biology , nucleic acid , dna , rna , thiocyanate , population , genetics , computational biology , biochemistry , gene , demography , sociology
The nucleic acids in field collected samples of viruses, bacteria, yeasts, invertebrates and vertebrates were well preserved in buffered solution of 4M guanidium‐thiocyanate. This method is particularly suitable for population, epidemiological and parasitological molecular studies. The DNA and RNA from samples kept up to 41 days without refrigeration in this way were suitable for PCR and reverse transcription PCR.