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Limitations of the RAPD technique in phylogeny reconstruction in Drosophila
Author(s) -
Van De Zande Louis,
Bijlsma R.
Publication year - 1995
Publication title -
journal of evolutionary biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.289
H-Index - 128
eISSN - 1420-9101
pISSN - 1010-061X
DOI - 10.1046/j.1420-9101.1995.8050645.x
Subject(s) - biology , rapd , phylogenetic tree , phylogenetics , evolutionary biology , genetics , homology (biology) , phylogenetic relationship , drosophila (subgenus) , gene , genetic diversity , population , demography , sociology
In this study the limitations of the RAPD technique for phylogenetic analysis of very closely related and less related species of Drosophila are examined. In addition, assumptions of positional homology of amplified fragments in different species are examined by cross‐hybridization of RAPD fragments. It is demonstrated that in Drosophila the use of RAPD markers is very efficient in identification of species. For assessment of phylogenetic relationships, however, the method is limited to sibling species, and reliable measures for genetic distances cannot be obtained. Hybridization experiments demonstrate that fragments of similar length amplified from different species are not always derived from corresponding loci, and that not all RAPD fragments within the same amplification pattern are independent.