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Over‐expressed rice ADP‐ribosylation factor 1 (RARF1) induces pathogenesis‐related genes and pathogen resistance in tobacco plants
Author(s) -
Lee Won Young,
Hong Joon Ki,
Kim Cha Young,
Chun Hyun Jin,
Park Hyeong Cheol,
Kim Jong Cheol,
Yun DaeJin,
Chung Woo Sik,
Lee SungHo,
Lee Sang Yeol,
Cho Moo Je,
Lim Chae Oh
Publication year - 2003
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1046/j.1399-3054.2003.00215.x
Subject(s) - elicitor , biology , pathogen , gene , pathogenesis related protein , salicylic acid , complementary dna , tobacco mosaic virus , inducer , gene expression , microbiology and biotechnology , genetics , virus
A cDNA encoding RARF1 ( r ice A DP‐ r ibosylation f actor 1 ) was isolated from fungal elicitor‐treated rice suspension culture cells by mRNA differential display. RARF1 transcripts accumulated in response to hydrogen peroxide (H 2 O 2 ) and salicylic acid (SA) and rapidly in cells inoculated with an avirulent pathogen. Constitutively over‐expressed RARF1 under the control of the cauliflower mosaic virus 35S promoter (CaMV 35S) triggered spontaneous induction of lesion mimics, induced an array of pathogenosis‐related (PR) genes, reduced susceptibility to a fungal pathogen, and caused accumulation of SA. From these data, we deduced that RARF1 might be a component of various plant defence signalling pathways involved in inducing the expression of a subset of PR genes.