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Fusicoccin stimulates the production of H 2 O 2 in sycamore cell cultures and induces alternative respiration and cytochrome c leakage from mitochondria
Author(s) -
Malerba Massimo,
Crosti Paolo,
Cerana Raffaella,
Bianchetti Renato
Publication year - 2003
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1046/j.1399-3054.2003.00194.x
Subject(s) - fusicoccin , alternative oxidase , mitochondrion , biochemistry , cytochrome c oxidase , activator (genetics) , atpase , enzyme , microbiology and biotechnology , biology , chemistry , biophysics , receptor
Fusicoccin (FC) is a well known toxin acting as a 14‐3‐3 protein‐mediated activator of the plasma membrane H + ‐ATPase and it has been widely used to study the regulatory mechanism and the physiological role of this enzyme's activity. Recently, FC has been shown to induce other responses similar to those occurring under a stress condition, perhaps not strictly dependent on the activation of proton extrusion. In this paper we report that in cultured sycamore ( Acer pseudoplatanus L.) cells FC induces H 2 O 2 overproduction as well as other novel, presumably related responses, such as the activation of the alternative oxidase and the leakage of cytochrome c from the mitochondria, accompanied by a decrease of the cytochrome pathway capacity. The relationship between H 2 O 2 production and other phenomena has also been studied by means of exogenously added H 2 O 2 .