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Molecular analysis of sequence variants in the Fcɛ receptor I β gene and IL‐4 gene promoter in Italian atopic families
Author(s) -
Rigoli L.,
Di Bella C.,
Procopio V.,
Barberio G.,
Barberi I.,
Caminiti L.,
La Grutta S.,
Briuglia S.,
Salpietro C. D.,
Pajno G. B.
Publication year - 2004
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1046/j.1398-9995.2003.00385.x
Subject(s) - atopy , allele , genotype , allergy , gene , genetics , biology , promoter , immunology , immunoglobulin e , gene polymorphism , restriction fragment length polymorphism , polymorphism (computer science) , microbiology and biotechnology , antibody , gene expression
Background: The genetic variants in the Fc ɛ receptor I β gene (Glu237Gly) and the T allele of the (C590T) polymorphism of interleukin (IL)‐4 gene promoter were reported to be associated with atopy. But the data of the studies in different populations are contrasting with one another. Methods: A group of 25 Italian nuclear families were studied. In each family at least two allergic subjects were present. The allergic children were 65 and the allergic relatives were 35. One hundred and three nonallergic unrelated controls included outpatiens with no history of atopy. The (C590T) promoter polymorphism of the IL‐4 and the genetic variant Glu237Gly of Fc ɛ RI β genes were analysed by the polymerase chain reaction‐restriction fragment length polymorphism method. Results: A significant difference was observed in the genotype frequency at codon 237 of the Fc ɛ RI β gene between allergic children and nonatopic control ( P < 0.01) and in the allergic relatives ( P < 0.001). In the children, the Glu237Gly polymorphism was also associated with elevated circulating levels of immunoglobulin E. The ‐590C/T allele of IL‐4 promoter gene showed no association with atopy. Conclusions: In our study, the Glu237Gly polymorphism of the Fc ɛ RI β gene was associated with atopy. Our results have not pointed out an association between the (C590T) promoter polymorphism of the IL‐4 gene and atopy. These data suggest the potential role of the Fc RI β gene in the development of the allergy.