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Reconstruction of epidermis by grafting of keratinocytes cultured on polymer support – clinical study
Author(s) -
Dvořánková Barbora,
Holíková Zuzana,
Vacík Jiří,
Königová Radana,
Kapounková Zuzana,
Michálek Jiří,
Přádn Martin,
Smetana Karel
Publication year - 2003
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1046/j.1365-4362.2003.01792.x
Subject(s) - epidermis (zoology) , basal lamina , medicine , cytokeratin , immunocytochemistry , laminin , wound healing , skin grafting , pathology , monoclonal antibody , keratinocyte , basal (medicine) , immunohistochemistry , anatomy , microbiology and biotechnology , biology , surgery , in vitro , antibody , extracellular matrix , ultrastructure , immunology , biochemistry , insulin
Background Extensive wound coverage still represents a challenge for contemporary medicine. We demonstrate the results of a clinical trial of the grafting of cultured keratinocytes directly on a polymer cultivation support in the treatment of skin defects in seriously burned patients and in patients with trophic ulcers. Methods Wound closure was evaluated clinically. The morphology and phenotypic pattern of the reconstructed epidermis, including the basal lamina, as well as the presence of Langerhans cells, were evaluated immunocytochemically using a panel of monoclonal antibodies. Results All layers of the reconstructed epidermis were normally differentiated (cytokeratin immunocytochemistry). The basal lamina contained collagen type IV and laminin. The reconstructed epidermis was extensively colonized by Langerhans cells. Conclusions The results of the described technology are encouraging, especially in patients after a burn injury. The described procedure is suitable for the treatment of skin defects in clinical practice.

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