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Modulation in vitro of keratinocyte integrins by interferon‐α and interferon‐γ
Author(s) -
Tenaud Isabelle,
Leroy Sabine,
Chebassier Nathalie,
Dreno Brigitte
Publication year - 2002
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1046/j.1365-4362.2002.01598.x
Subject(s) - keratinocyte , integrin , alpha interferon , interferon , interferon gamma , in vitro , flow cytometry , microbiology and biotechnology , immunology , biology , cytokine , cell , biochemistry
Abstract Background Interferon‐α and ‐γ are glycoproteins with antiviral and immunoregulatory properties. In vitro studies have shown a role for these cytokines in the regulation of epidermal keratinocyte growth and differentiation. In the same way, integrins are adhesion molecules which regulate keratinocyte proliferation and differentiation. Aim To determine whether the regulatory activity of interferons on keratinocyte proliferation and differentiation is related to a modulation of keratinocyte integrins. Methods Two different methods were used: monolayers and reconstituted skin, incubated either with 1200 U/mL interferon‐α or 500 U/mL interferon‐γ or control medium for 48 h. The integrin expression was assessed by flow cytometry and immunohistochemistry. Results In monolayers, only the α3 subunit was significantly inhibited by interferon‐γ. In reconstituted skin, where keratinocytes are differentiated, both interferons had an inductive effect on β1 expression and interferon‐α had an inhibitory effect on α6 expression. Conclusion Interferon‐α and ‐γ induce a modulatory effect on α3, α6 and β1 which appears to be related to the state of differentiation. Moreover, the decreased expression of α6 and α3 could be one of the mechanisms involved in the formation of bullous lesions during long‐term interferon therapy.