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Effect of palmitic acid on neutrophil functions in vitro
Author(s) -
Akamatsu Hirohiko,
Niwa Yukie,
Matsunaga Kayoko
Publication year - 2001
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1046/j.1365-4362.2001.01292.x
Subject(s) - palmitic acid , xanthine oxidase , superoxide , reactive oxygen species , phagocytosis , linoleic acid , respiratory burst , biochemistry , inflammation , chemistry , medicine , immunology , fatty acid , enzyme
Background It has been shown that in acne comedones the proportion of linoleic acid is markedly decreased, while palmitic acid is significantly increased. We previously reported that the decreased proprtion of linoleic acid, which markedly suppresses neutrophil reactive oxygen species (ROS) generation and phagocytosis, contribute to the worsening of acne inflammation. The aim of this study was to examine the effect of palmitic acid on neutrophil functions in vitro . Methods We investigated the effect of palmitic acid on inflammatory parameters such as neutrophil chemotaxis, phagocytosis, and ROS generation. Reactive oxygen species generation in a cell‐free, xanthine‐xanthine oxidase system was also assessed. The species examined were superoxide radical anion (O 2 – ), hydrogen peroxide (H 2 O 2 ), and hydroxyl radical (OH·). Results Palmitic acid significantly decreased H 2 O 2 generation both by neutrophils and in the xanthine‐xanthine oxidase system, while neutrophil chemotaxis and phagocytosis as well as O 2 – and OH· generation by both systems were not markedly affected in the presence of palmitic acid. Conclusions The present study suggests that palmitic acid may be involved in the pathogenesis of acne inflammation from a standpoint of oxidative tissue injury.