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Orobanche species and population discrimination using intersimple sequence repeat (ISSR)
Author(s) -
Benharrat H,
Veronesi C,
Theodet C,
Thalouarn P
Publication year - 2002
Publication title -
weed research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 74
eISSN - 1365-3180
pISSN - 0043-1737
DOI - 10.1046/j.1365-3180.2002.00305.x
Subject(s) - biology , intraspecific competition , orobanche , polymerase chain reaction , primer (cosmetics) , genetic variation , genetic diversity , polymorphism (computer science) , genetics , population , botany , evolutionary biology , allele , zoology , gene , chemistry , germination , demography , organic chemistry , sociology
Summary Orobanche species are commonly identified using morphological characteristics. In many cases, the distinction of closely related species is difficult, and a molecular tool is more suitable to differentiate them. In this study, genomic polymorphism between morphologically distinct species was investigated through amplification by polymerase chain reaction (PCR) of intersimple sequence repeat (ISSR) regions. Five primers were used to study genetic variation in the morphologically distinct species O. hederae and O. amethystea , as well as the closely related species O. cernua and O. cumana . For the first two species, all the primers detected genetic polymorphism. Anchored primers allowed the identification of more specific molecular markers than non‐anchored tri‐ and tetranucleotide primers. Genetic polymorphism was investigated among three O. hederae populations using the two types of primer. One non‐anchored and two anchored primers detected intraspecific variation, which was not correlated with the geographical location of those populations. The primer (GATA) 4 detected polymorphism between five specimens each of O. cernua and O. cumana species collected from different countries, permitting these two closely related species to be clearly differentiated. This study demonstrated that ISSR markers can be highly reliable for precise identification of Orobanche species.