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Resistance to diclofop‐methyl in two Lolium spp. populations from Italy: studies on the mechanism of resistance
Author(s) -
Bravin F,
Zanin G,
Preston C
Publication year - 2001
Publication title -
weed research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 74
eISSN - 1365-3180
pISSN - 0043-1737
DOI - 10.1046/j.1365-3180.2001.00250.x
Subject(s) - metabolism , biology , population , phytopharmacology , acetyl coa carboxylase , pesticide resistance , pyruvate carboxylase , poaceae , biochemistry , botany , pesticide , enzyme , agronomy , medicine , environmental health
The mechanisms of herbicide resistance were investigated in two diclofop‐methyl‐resistant Lolium spp. populations from central Italy, Roma ’94 and Tuscania ’97. These two populations were compared with two susceptible Italian populations (Vetralla ’94, Tarquinia ’97) and a resistant and a susceptible population from Australia, SLR31 and VLR1. The activity of acetyl Co‐A carboxylase (ACCase) extracted from susceptible (S) or resistant (R) individuals from the Italian populations was inhibited by both aryloxyphenoxypropanoate (diclofop acid and fluazifop acid) and cyclohexanedione (sethoxydim) herbicides. Diclofop‐methyl was rapidly de‐esterified to diclofop acid at a similar rate in both R and S populations. In all populations, diclofop acid was subsequently degraded to other metabolites. The rate of degradation of diclofop acid was not significantly faster in R than in S populations; however, diclofop acid was degraded more completely in Roma ’94 and Tuscania ’97 compared with the S populations. Application of the mixed‐function oxidase inhibitor 1‐aminobenzotriazole (ABT) significantly enhanced diclofop‐methyl toxicity towards both R populations, but not in S populations. However, enhanced herbicide metabolism does not completely account for the measured resistance level. A mechanism other than an altered ACCase and enhanced herbicide metabolism appears to be responsible for resistance to diclofop‐methyl in Roma ’94 and Tuscania ’97.