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Comparison of sulfonylurea herbicide residue detection in soil by bioassay, enzyme‐linked immunosorbent assay and hplc
Author(s) -
Hollaway,
Kookana,
McQuinn,
Moerkerk,
Noy,
Tomasz Smal
Publication year - 1999
Publication title -
weed research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 74
eISSN - 1365-3180
pISSN - 0043-1737
DOI - 10.1046/j.1365-3180.1999.00153.x
Subject(s) - bioassay , sulfonylurea , chromatography , chemistry , high performance liquid chromatography , residue (chemistry) , immunoassay , environmental chemistry , biochemistry , biology , microbiology and biotechnology , antibody , genetics , insulin , immunology
The ability of bioassay, enzyme‐linked immunosorbent assay (ELISA) and high‐performance liquid chromatography (hplc) methods to detect sulfonylurea herbicides in soil was evaluated as part of a project studying the leaching and persistence of these herbicides in the alkaline soils of south‐eastern Australia. Soil samples with known concentrations between 0.1 and 10 μg a.i. kg −1 chlorsulfuron, metsulfuron‐methyl or triasulfuron were prepared by an independent laboratory and supplied in coded bags to separate laboratories for testing. The accuracy of the results was analysed, and the merits of each method are discussed. Bioassay was suitable for measuring biologically active residues from 0.1 to 1.0 μg a.i. kg −1 . ELISA accurately measured residues in the range of 0.1–10 μg a.i. kg −1 , making it the most widely adaptable assay tested. It will be useful for measuring residues in sodic subsoils where bioassay plants grow poorly. There was good reproducibility between the bioassay and ELISA. The hplc technique used in this study was not as accurate as bioassay or ELISA at quantifying residues of 3.0–10 μg a.i. kg −1 and could not detect residues at or below 1.0 μg a.i. kg −1 .