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A molecular genetic assessment of herbicide‐resistant Sinapis arvensis
Author(s) -
A. Meikle,
R. P. Finch,
Neil McRoberts,
G. Marshall
Publication year - 1999
Publication title -
weed research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 74
eISSN - 1365-3180
pISSN - 0043-1737
DOI - 10.1046/j.1365-3180.1999.00132.x
Subject(s) - biology , weed , population , dicamba , loss of heterozygosity , sulfonylurea , dna profiling , botany , genetics , dna , weed control , agronomy , gene , allele , microbiology and biotechnology , demography , sociology , insulin
The acquisition of resistance to both the auxinic herbicide dicamba and the sulfonylurea herbicide chlorsulfuron has been recorded in Canadian populations of the weed species Sinapis arvensis L. (charlock, wild mustard) To study the effect of this selection for herbicide resistance on levels of genetic variation, polymerase chain reaction‐based DNA fingerprinting techniques were used to characterize two herbicide‐resistant and one susceptible population of S. arvensis . Analysis of the resultant DNA marker profiles revealed extensive polymorphism between individuals. However, segregation of the three biotypes was detectable despite high levels of intrabiotype polymorphism. No reduction in the levels of heterozygosity within the resistant populations were found compared with the susceptible population.