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A rapid reliable test for screening aryloxyphenoxy‐ propionic acid resistance within Alopecurus myosuroides and Lolium spp. populations
Author(s) -
Anne Letouzé,
Jacques Gasquez
Publication year - 1999
Publication title -
weed research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 74
eISSN - 1365-3180
pISSN - 0043-1737
DOI - 10.1046/j.1365-3180.1999.00120.x
Subject(s) - coleoptile , biology , lolium , seedling , poaceae , bioassay , agronomy , lolium perenne , botany , horticulture , ecology
A reliable seedling bioassay was developed and tested for the rapid screening for resistance to aryloxyphenoxypropionic (APP) herbicides in Alopecurus myosuroides and Lolium spp. populations. It is based upon the difference in coleoptile length of resistant and susceptible A. myosuroides and Lolium seedlings, respectively, exposed to fenoxaprop‐P acid and diclofop acid solution for 6 days in a plastic box. A 6 mg L −1 fenoxaprop‐P acid solution was selected as the best concentration for a reliable screening of resistant biotypes within A. myosuroides populations. At this concentration, coleoptile lengths of susceptible and resistant seedlings were shorter and longer than 10 mm respectively. Similarly, resistant seedlings within Lolium populations were easily detected at 10 mg L −1 diclofop acid. At this concentration, coleoptile lengths of susceptible and resistant seedlings were shorter and longer than 20 mm respectively. For both populations, the coleoptile length distributions appear to discriminate between two kinds of APP‐resistant biotypes (highly and slightly resistant).