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Response of Brassica napus in tissue culture to metsulfuron‐methyl and chlorsulfuron
Author(s) -
Seiden,
Kappel,
Streibig
Publication year - 1998
Publication title -
weed research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.693
H-Index - 74
eISSN - 1365-3180
pISSN - 0043-1737
DOI - 10.1046/j.1365-3180.1998.00091.x
Subject(s) - brassica , sulfonylurea , bioassay , chemistry , dilution , matrix (chemical analysis) , chromatography , botany , biology , microbiology and biotechnology , physics , insulin , genetics , thermodynamics
A herbicide bioassay based on tissue cultures of Brassica napus L. was evaluated with selected sulfonylurea herbicides. Data were analysed by fitting the results to a log‐logistic dose–response model. Within an experiment, the non‐linear regression models were fitted simultaneously to the individual dose–response curves. The results obtained showed good response to even low concentrations of herbicide, with detection limits in the range 0.008–0.69 nmol L −1 for chlorsulfuron and 0.02–0.13 nmol L −1 for metsulfuron. The reproducibility of the assays, on the basis of coefficient of variation of the ED 50 values, was found to be 44% for chlorsulfuron and 48% for metsulfuron measurements. Assay of herbicide dissolved in aqueous soil extract showed significant interference from this matrix on the response, requiring a five times dilution of the extract to overcome this matrix effect.