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Influence of vitamin E on mast cell mediator release
Author(s) -
Gueck Thomas,
Aschenbach Jörg Rudolph,
Fuhrmann Herbert
Publication year - 2002
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1046/j.1365-3164.2002.00307.x
Subject(s) - histamine , chymase , mastoparan , chemistry , prostaglandin d2 , mediator , vitamin , endocrinology , histamine h4 receptor , vitamin e , mast cell , tryptase , medicine , vitamin d and neurology , prostaglandin , immunology , biochemistry , antioxidant , histamine h2 receptor , receptor , g protein , antagonist
We investigated the influence of vitamin E on mediator activity and release in a canine mastocytoma cell line (C2) as a model for canine atopic dermatitis. Cells were incubated without and with vitamin E (100 µ m ) for 24 h. The histamine and prostaglandin D 2 (PGD 2 ) release as well as the chymase and tryptase activity were measured. To stimulate the PGD 2 and histamine release, cells were incubated with the wasp venom peptide mastoparan (50 µ m ) for 30 or 45 min. Nonstimulated as well as mastoparan‐stimulated histamine and PGD 2 release was reduced significantly in vitamin E‐treated cells. The activity of chymase tended to decrease, but the tryptase activity of C2 cells was not influenced by vitamin E. These results indicate that vitamin E decreased the production and release of inflammatory mediators in C2 cells, suggesting that vitamin E might have a possible beneficial effect in inflammatory diseases.