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Comparison of three monoclonal and three polyclonal antibodies in the immunohistochemical diagnosis of canine autoimmune skin diseases
Author(s) -
Pérez José,
Arce Cristina,
Moreno Angela,
Mozos Elena,
Rodríguez Francisco,
Llanes Diego
Publication year - 2002
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1046/j.1365-3164.2002.00306.x
Subject(s) - polyclonal antibodies , immunohistochemistry , monoclonal antibody , medicine , pemphigus vulgaris , monoclonal , pathology , discoid lupus erythematosus , antibody , immunology , lupus erythematosus , pemphigus , pemphigus foliaceus , autoantibody
The aim of this study was to evaluate the utility of three monoclonal antibodies (mAbs), and two anticanine IgG and one anticanine IgM polyclonal antibodies (pAbs) for the immunohistochemical diagnosis of canine autoimmune skin diseases. Skin biopsies from 11 cases of pemphigus (7 foliaceus, 3 vulgaris and 1 erythematosus), 12 cases of discoid lupus erythematosus (DLE) and 12 cases of chronic hyperplasic dermatitis were used. The CA4E7 mAb (IgG 1 + IgG 2 ) showed similar sensitivity, but higher specificity and lower background than the two anti‐IgG pAbs for the immunohistochemical diagnosis of pemphigus and DLE. The CA4F1 mAb (IgG 2 ) and CA3H1 mAb (IgG 2 ) showed moderate and low interepithelial reactivity, respectively, in autoimmune skin diseases, but strong staining of the cytoplasm of plasma cells of the inflammatory infiltrates. These results suggest that the CA4E7 mAb may be valuable in the immunohistochemical diagnosis of such disorders.