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Comparative sequence analysis of three canine type 2 keratins
Author(s) -
Credille K. M.,
Murphy K. E.,
Venta P. J.,
Page L.,
Harville M. A.,
Tucker K. A.,
Miller A. B.,
Mruthyunjaya A.,
Barnhart K. A.,
Dunstan R. W.
Publication year - 2002
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1046/j.1365-3164.2002.00298_8.x
Subject(s) - biology , intron , gene , homology (biology) , exon , genetics , microbiology and biotechnology , complementary dna , genomic dna , keratin , amino acid , peptide sequence , oligonucleotide , sequence alignment , sequence analysis
In order to better define the cornification process in dogs and to understand how it is similar or different from cornification in other mammals, we are sequencing the major molecules of cornification in the dog. In this report, we describe the complete genomic sequence of three superficial type 2 canine keratins: K1, K2e and K2p. Oligonucleotide primers for PCR were designed from homologous regions of previously reported human or murine cDNA sequences of the genes of interest. DNA sequencing and evaluation with blast confirmed that the primers amplified the respective canine genes. These primers were then used to screen a subset of a canine genomic library known to contain basic keratin genes. Selected clones were sequenced to obtain the complete DNA sequence. K1, K2e and K2p each had nine exons and eight introns characteristic of the type 2 keratins that code for proteins with variable glycine‐rich head and tail regions and a central α‐helical rod domain. K2p encoded a protein of 659 amino acids, larger than both K1 and K2e (620 and 634 amino acids, respectively). Amino acid sequence homology with humans was 81, 74 and 81% for K1, K2e and K2p, respectively, with homology greatest at the central rod region and decreasing dramatically at the head and tail. Comparisons of the amino acid sequences of these keratins within the canine species defined K2e and K2p as having a slightly higher degree of similarity. In contrast, in humans K2e has greater sequence homology with K1. This study was funded in part by the Morris Animal Foundation.