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The effects of dietary n‐3 vs. n‐6 fatty acids on ex‐vivo LTB 4 generation by canine neutrophils
Author(s) -
Byrne Kevin P.,
Campbell Karen L.,
Davis Carol A.,
Schaeffer David J.,
Troutt Fred H.
Publication year - 2000
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1046/j.1365-3164.2000.00171.x
Subject(s) - polyunsaturated fatty acid , ex vivo , fatty acid , linoleic acid , chemistry , triglyceride , arachidonic acid , biochemistry , cholesterol , in vitro , enzyme
Dietary n‐3 polyunsaturated fatty acids are widely used for amelioration of inflammatory skin disease in dogs. In this study, a diet containing two different sources of n‐3 polyunsaturated fatty acid–triglyceride (from menhaden oil) and concentrated ethyl esters–was fed to one group of six purpose‐bred dogs, while an isocaloric isonitrogenous diet with corn oil (n‐6 polyunsaturated fatty acids) was fed to another group of eight purpose‐bred dogs for six weeks. Peripheral blood neutrophils, isolated at week–1 (baseline), week 2 and week 6, were stimulated with calcium ionophore A23187 and the amount of leukotriene B 4 produced was determined via reversed‐phase high performance liquid chromatography. Analysis of variance of log‐transformed data revealed a significant effect for diet ( P = 0.005) at six weeks, with dogs fed the highn‐3 polyunsaturated fatty acid diet having significantly less mean ex vivo neutrophil leukotriene B 4 production than dogs fed the high n‐6 polyunsaturated fatty acid diet. Further studies on the clinical usefulness of n‐3 polyunsaturated fatty acid ethyl esters are warranted.