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Canine epidermolysis bullosa acquisita: circulating autoantibodies target the aminoterminal non‐collagenous (NC1) domain of collagen VII in anchoring fibrils
Author(s) -
OLIVRY THIERRY,
FINE JODAVID,
DUNSTON STANLEY M.,
CHASSE DAWN,
TENORIO AUREA PASCAL,
MONTEIRORIVIERE NANCY A.,
CHEN MEI,
WOODLEY DAVID T.
Publication year - 1998
Publication title -
veterinary dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.744
H-Index - 60
eISSN - 1365-3164
pISSN - 0959-4493
DOI - 10.1046/j.1365-3164.1998.00067.x
Subject(s) - epidermolysis bullosa acquisita , anchoring fibrils , autoantibody , pathology , immunoelectron microscopy , epidermolysis bullosa , medicine , cicatricial pemphigoid , pemphigoid , dermis , basement membrane , bullous pemphigoid , immunology , antibody , immunohistochemistry
The classification of autoimmune blistering skin diseases is based on the skin antigen(s) targeted by pathogenic autoantibodies. In humans and dogs, there is increasing evidence that autoimmune subepidermal bullous diseases represent different nosological entities. This study establishes the existence of the canine equivalent of epidermolysis bullosa acquisita (EBA) in humans. Canine EBA, like the inflammatory variant of its human counterpart, is characterized by spontaneous vesicles arising from an inflammatory eruption. Dermo‐epidermal separation occurs in association with neutrophilic infiltration in the superficial dermis. Tissue‐fixed and circulating IgA and IgG autoantibodies specific for the lower basement membrane zone can be detected by immunofluorescence methods. Using immunoelectron microscopy, autoantibodies are shown to target the distal end of anchoring fibrils in the sublamina densa. ELISA and immunoblotting utilizing eukaryotically expressed recombinant collagen VII subdomains confirm that the circulating autoantibodies are specific for the aminoterminal globular non‐collagenous NC1 domain of type VII collagen.