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Short communication: Erythrocyte binding antigen (EBA‐175) of Plasmodium falciparum : improved genotype determination by nested polymerase chain reaction
Author(s) -
Touré Fousseyni S.,
Mavoungou Elie,
Me Ndong Jérôme Mezui,
Tshipamba Paul,
Deloron Philippe
Publication year - 2001
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1046/j.1365-3156.2001.00789.x
Subject(s) - plasmodium falciparum , polymerase chain reaction , nested polymerase chain reaction , genotype , biology , microbiology and biotechnology , allele , antigen , virology , gene , malaria , genetics , immunology
We have designed primers to the conserved region of the erythrocyte binding antigen (EBA)‐175 gene which amplify specifically the two alleles by polymerase chain reaction (PCR) and by nested PCR. This approach provides a specific, sensitive and rapid method for genotype determination in a large number of Plasmodium falciparum field isolates.