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Genotypic patterns of multiple isolates of M. tuberculosis from tuberculous HIV patients
Author(s) -
Lourenço M. C. S.,
Grinsztejn B.,
FandinhoMontes F. C. O.,
Silva M. G.,
Saad M. H. F.,
Fonseca L. S.
Publication year - 2000
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1046/j.1365-3156.2000.00583.x
Subject(s) - tuberculosis , genotype , virology , human immunodeficiency virus (hiv) , mycobacterium tuberculosis , biology , medicine , genetics , pathology , gene
Summary We investigated whether the recurrence of tuberculosis in HIV‐infected patients is due to an exogenous reinfection or relapses after antituberculosis chemotherapy. We reviewed clinical information on 32 patients at a Rio de Janeiro hospital from whom multiple Mycobacterium tuberculosis isolates were taken. All isolates were analysed by DRE‐PCR fingerprinting technique, and those with identical DRE‐PCR patterns were analysed by the RFLP method. Twenty patients had M. tuberculosis simultaneously isolated from different organs. These patients and nine others with sequential positive cultures after 2 months of therapy showed stable DRE‐PCR and RFLP patterns. One patient's isolate became resistant to isoniazid, but the molecular pattern remained unchanged despite the development of drug resistance. In three patients, the DRE‐PCR patterns of the isolates changed dramatically. Clinical and microbiological evidence was consistent with active tuberculosis caused by a new strain of M. tuberculosis . The exogenous reinfection of the three patients was not due to an outbreak, but the isolates from each patient showed unique patterns.