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Population structure of recrudescent Plasmodium falciparum isolates from Western Uganda
Author(s) -
Jelinek T.,
Kilian A. H. D,
Westermeier A.,
Pröll S.,
Kabagambe G.,
Nothdurft H. D.,
Sonnenburg F.,
Löscher T.
Publication year - 1999
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1046/j.1365-3156.1999.00428.x
Subject(s) - genotyping , plasmodium falciparum , biology , malaria , drug resistance , virology , merozoite surface protein , genotype , population , microbiology and biotechnology , genetics , gene , medicine , immunology , environmental health , malaria vaccine
Summary It has been proposed that polymorphisms of the Merozoite Surface Protein 1 and 2 (MSP1 and MSP2) and the Glutamate Rich Protein (GLURP) genes can be considered as genetic markers for the genotyping of field populations of Plasmodium falciparum . During a field study on in vivo drug resistance against chloroquine, sulphadoxine/pyrimethamine (S/P) and cotrimoxazole in West Uganda, sensitive and resistant isolates were collected from patients by fingerprick for genotyping. 59 (72.8%) of the 81 P. falciparum samples isolated at day 0 showed multiclonal infection with 2–7 clones. Among the isolates we investigated, presence of the allelic family MAD20 of MSP1 at day 0 was significantly ( P = 0.0041) associated with decreased resistance to antimalarials. Use of this method in a field study on in vivo drug resistance demonstrates another potential application of genotyping as a tool for epidemiological investigations.