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Five subgroups of Blastocystis hominis isolates from symptomatic and asymptomatic patients revealed by restriction site analysis of PCR‐amplified 16S‐like rDNA
Author(s) -
BöhmGloning B.,
Knobloch J.,
Walderich B.
Publication year - 1997
Publication title -
tropical medicine and international health
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.056
H-Index - 114
eISSN - 1365-3156
pISSN - 1360-2276
DOI - 10.1046/j.1365-3156.1997.d01-383.x
Subject(s) - biology , restriction fragment length polymorphism , restriction enzyme , microbiology and biotechnology , polymerase chain reaction , virology , dna , gene , genetics
DNA polymorphism of Blastocystis hominis isolates was examined by the amplification of a gene fragment coding for the 16S‐like rRNA. Using identical primers, fragments of approximately 850 bp were amplified from 110 B. hominis isolates and fragments of 1.1kbp were amplified from 48 isolates. Digestion of the amplification products with the restriction enzymes Hin fI, Rsa I, and Alu I revealed different profiles for each fragment length. Subgroup I and II, resulting from digestion of the smaller 850 bp fragment, have identical Hin fI and Alu I restriction bands, subgroup III and IV have identical Rsa I fragments after digestion of the 1.1kbp DNA. Subgroup V resembles subgroup III in a few bands after the Rsa I and Alu I restriction, respectively. Ninety (54%) of the isolates studied were assigned to subgroup I, 20 (12%) to subgroup II, 35 (21%) to subgroup III, 12 (7%) to subgroup IV, and 1 (1%) to subgroup V. Five (3%) of the examined people were coinfected with B. hominis of subgroup I and III, 3 (2%) carried B. hominis of subgroup I and II. These results show that there are 5 B. hominis subgroups none of which was found to be significantly correlated with the reported disease.