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Human neutrophil antigen‐4a gene frequencies in an Australian population, determined by a new polymerase chain reaction method using sequence‐specific primers
Author(s) -
Clague H. D.,
Fung Y. L.,
Minchinton R. M.
Publication year - 2003
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1046/j.1365-3148.2003.00435.x
Subject(s) - genotyping , polymerase chain reaction , primer (cosmetics) , genotype , biology , antigen , population , gene , immunology , microbiology and biotechnology , virology , genetics , medicine , chemistry , environmental health , organic chemistry
Summary. Human neutrophil antigen‐4a (HNA‐4a) is a high‐frequency (99% in the USA) neutrophil antigen, which has recently been linked to a case of alloimmune neonatal neutropenia. We have devised a new polymerase chain reaction sequence‐specific primer (PCR‐SSP) method to assess HNA‐4a genotype, and used it to determine the HNA‐4a gene frequencies in an Australian population. The gene frequency was found to be 0·906, which is the same as in the American population. The PCR‐SSP genotyping method perfectly correlates with serological phenotyping and is efficient for screening large numbers of samples.