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Used leucodepletion filters as a source of large quantities of DNA suitable for the study of genetic variations in human populations
Author(s) -
Cook M. A.,
Jobson S. E.,
Atkinson D. C.,
Lowe D. P.,
Farmer S. L.,
AlviAli W. J.,
Smith N. A.,
Briggs D. C.
Publication year - 2003
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1046/j.1365-3148.2003.00423.x
Subject(s) - polymerase chain reaction , dna , genomic dna , dna profiling , biology , computational biology , genetics , population , medicine , gene , environmental health
summary . A simple technique for developing large control panels with large quantities of DNA suitable for studies in population genetics was established. Background and objectives Both a lack of suitable controls and insufficient quantities of DNA for repeated analysis of the same control group often hamper the investigation of genetic markers for disease. Materials and methods Using a waste product from routine blood donation, we describe a simple method that allows the investigator to extract large amounts of DNA. Results A mean of 1520 µg of DNA per sample was obtained. The DNA obtained remains suitable for polymerase chain reaction and sequencing techniques after 2 years of storage at both 4 °C and −40 °C. Conclusion This technique allows the development of a large panel of controls with sufficient quantities of genomic DNA for thousands of tests.