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A new murine monoclonal antibody against Kx protein
Author(s) -
Hattab C.,
Blanchard D.,
Gane P.,
Verkarre V.,
PetitLeroux Y.,
Loirat M.J.,
Cartron J.P.,
Bertrand O.
Publication year - 2003
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1046/j.1365-3148.2003.00414.x
Subject(s) - monoclonal antibody , western blot , flow cytometry , microbiology and biotechnology , blot , antibody , calpain , immunoprecipitation , red blood cell , epitope , biology , intracellular , chemistry , immunology , biochemistry , gene , enzyme
Summary . Mice immunized with a synthetic peptide located on an intracellular segment of the polytopic Kx protein (37 kDa) from human red blood cells (RBCs) produced a monoclonal antibody called C7B8. As expected, this antibody did not agglutinate common RBCs but reacted with permeabilized cells in flow cytometry. C7B8 recognizes the Kx protein on Western blots. Cross‐reactivity of C7B8 with human calpain of human muscle extracts was demonstrated by Western blot analysis. This cross‐reactivity precludes the use of C7B8 for Kx tissue distribution studies, but immobilized C7B8 was a convenient tool for purification of the Kell–Kx complex from RBC membrane extract by immunochromatography.