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Harvesting of CD34 antigen‐expressing cells with a new programme for the collection of mononuclear cells with use of the Amicus (Baxter) blood cell separator
Author(s) -
Moog R.
Publication year - 2002
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1046/j.1365-3148.2002.00405.x
Subject(s) - peripheral blood mononuclear cell , cd34 , medicine , blood product , apheresis , blood donor , peripheral blood , body weight , stem cell , surgery , gastroenterology , andrology , immunology , platelet , chemistry , biology , in vitro , biochemistry , genetics
Summary A study was performed to evaluate a new programme for peripheral blood stem cell (PBSC) collection with the use of the Amicus (Baxter) blood cell separator. Healthy donors ( n = 9) and oncology patients ( n = 21) scheduled for PBSC transplant were studied. Ten PBSC harvests were performed in the donors and 30 in the patients. A median of 6·37 × 10 6 CD34 + cells per kg recipient body weight (range 3·08–11·06 × 10 6 ) were collected from the donors in a product weight of 169·5 g (118–186). From the patients, 6·26 × 10 6 CD34 + cells per kg body weight (range 0·2–53·6 × 10 6 ) were harvested in a product weighing 121·5 g (range 92–190). The median platelet contamination was 0·93 × 10 11 (range 0·45–1·23 × 10 11 ) per donor product and 0·2 × 10 11 (range 0·05–0·86 × 10 11 ) per patient product. No severe side effects were observed during or after the PBSC collection procedures.