Premium
Measurement of blood volume after haemodilution with haemoglobin‐based oxygen carriers by a radiolabelled‐albumin method
Author(s) -
Caron A.,
Mayer J.C.,
Menu P.,
Alayash A.,
Marie P.Y.,
Vigneron C.
Publication year - 2001
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1046/j.1365-3148.2001.00337.x
Subject(s) - hydroxyethyl starch , oncotic pressure , blood volume , blood substitute , albumin , dextran , hemoglobin , shock (circulatory) , anesthesia , chemistry , resuscitation , red blood cell , blood pressure , hemolysis , medicine , biochemistry
. Recent studies have shown that the use of haemoglobin‐based oxygen‐carrying solutions (HBOCs) for perioperative haemodilution could significantly reduce the need for packed red blood cells in clinical practice. Though the effects of HBOCs on plasma volume have been characterized in experimental models of volume resuscitation from hypovolaemic shock, little is known about their action in normovolaemic haemodilution conditions. We therefore applied a radiolabelled serumalbumin method to determine blood volume after haemodilution with crosslinked or conjugated haemoglobin, in comparison with a reference solution of hydroxyethyl starch (HES). Three groups of New Zealand white rabbits were studied ( n = 7 each group) subjected to moderate exchange transfusion with low molecular weight HES, bis(3,5‐dibromosalicyl)fumarate crosslinked haemoglobin (αα‐Hb), or dextran‐conjugated haemoglobin (Hb‐Dex‐BTC). HES induced no changes in heart rate and blood pressure. The amplitude and duration of blood pressure increase and bradycardia were similar in both haemoglobin groups. A significant contraction of blood volume (12%) was observed 60 min after haemodilution with αα‐Hb, compared to HES and Hb‐Dex‐BTC. At the same time point, a decrease in absolute haemoglobin (plasma haemoglobin × plasma volume) was also noted. This study suggests that in haemodilution conditions, the specific oncotic properties and circulating persistence of crosslinked and conjugated haemoglobin solutions affect the pattern of blood volume distribution differently.