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The frequency of anti‐C + anti‐G in the absence of anti‐D in alloimmunized pregnancies
Author(s) -
Palfi M.,
Gunnarsson C.
Publication year - 2001
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1046/j.1365-3148.2001.00306.x
Subject(s) - polyethylene glycol , antibody , pregnancy , peg ratio , medicine , immunology , chemistry , chromatography , andrology , biology , biochemistry , finance , economics , genetics
. Anti‐D+C are often initially identified in sera from alloimmunized women. Anti‐G may be present in these samples, mimicking anti‐D+C, and therefore the differentiation of anti‐D, ‐C and ‐G may be important. Sera from 27 alloimmunized women, initially identified as containing anti‐D + anti‐C, were analysed by adsorption/elution studies in the presence of polyethylene glycol (PEG) using R 0 r (D+C–G+) and r'r(D–C+G+) red blood cells (RBC). Additionally, 15/27 samples were tested by adsorption in the presence of PEG and subsequently warm elution, using r G r (D–C–G+) RBC. Anti‐G + anti‐C, without anti‐D, were identified in 4/27 samples (14·8%) and none of the newborn children needed postpartum treatment. The combination of D+G, D+C and D+C+G antibodies occurred in 25·9%, 11·1% and 48·1% of the women, respectively. Overall, anti‐G was detected in 24/27 samples (88·9%). Pregnant women shown to have anti‐G+C but not anti‐D should receive Rh immune globulin. Additionally, the finding of apparent anti‐D+C during pregnancy in D‐negative spouses may lead to paternity testing and therefore a correct antibody identification is necessary.