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Further analysis of D el (D‐elute) using polymerase chain reaction (PCR) with RHD gene‐specific primers
Author(s) -
Fukumori Y.,
Hori Y.,
Ohnoki S.,
Nagao N.,
Shibata H.,
Okubo Y.,
Yamaguchi H.
Publication year - 1997
Publication title -
transfusion medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.471
H-Index - 59
eISSN - 1365-3148
pISSN - 0958-7578
DOI - 10.1046/j.1365-3148.1997.d01-31.x
Subject(s) - polymerase chain reaction , microbiology and biotechnology , gene , biology , genetics
D el (D‐elute) in the Rh blood group system is a variant with very weak D antigen and no agglutination is found by the indirect antiglobulin test. This variant is characterized by the presence of anti‐D eluate obtained after an adsorption‐elution test using anti‐D antibodies. We studied here the molecular genetic status of D el by using polymerase chain reaction with sequence‐specific primers (PCR‐SSP). We screened 306 serologically apparent D‐negative Japanese donors comprising 102 D el types for exons 7, 4 and 10 of the RHD gene. No PCR product was found in all 204 non‐D el samples from the D‐seronegative donors. However, PCR products were found in all 102 D el samples and all 70 D‐seropositive samples tested by the three PCR methods for exons 7, 4 and 10 analysis. D el was found with CCee, CcEe and Ccee, but not with CCEe, CcEE, ccEE, ccEe or ccee phenotype. The incidences of D el in the samples with the serological phenotypes CCee, CcEe and Ccee were 80.0% (4/5), 68.2% (45/66) and 61.6% (53/86), respectively. The results provide evidence that D el samples have exons 4, 7 and 10 of an RHD gene present in some form. This is consistent with the evidence that D antigen is present on the cells although only detected by antibody adsorption and elution. The PCR‐SSP method in the present study is useful to confirm D el among serologically apparent D‐negative samples.