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ydk1‐D , an auxin‐responsive GH3 mutant that is involved in hypocotyl and root elongation
Author(s) -
Takase Tomoyuki,
Nakazawa Miki,
Ishikawa Akie,
Kawashima Mika,
Ichikawa Takanari,
Takahashi Naoki,
Shimada Hiroaki,
Manabe Katsushi,
Matsui Minami
Publication year - 2004
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2003.01973.x
Subject(s) - auxin , arabidopsis , hypocotyl , mutant , biology , gene , elongation , microbiology and biotechnology , lateral root , genetics , botany , materials science , ultimate tensile strength , metallurgy
Summary To study the GH3 gene family of Arabidopsis , we investigated a flanking sequence database of Arabidopsis activation‐tagged lines. We found a dwarf mutant, named y a d o k ari 1‐D ( ydk1‐D ), that had a T‐DNA insertion proximal to a GH3 gene. ydk1‐D is dominant and has a short hypocotyl not only in light but also in darkness. Moreover, ydk1‐D has a short primary root, a reduced lateral root number, and reduced apical dominance. A GH3 gene, named YDK1 , was upregulated in ydk1‐D , and YDK1 transgenic plants showed the ydk1‐D phenotype. YDK1 gene expression was induced by exogenously applied auxin and regulated by auxin‐response factor (ARF)7. In addition, YDK1 gene expression was downregulated by blue and far‐red (FR) lights. Strong promoter activity of YDK1 was observed in roots and flowers. These results suggest that YDK1 may function as a negative component in auxin signaling by regulating auxin activity.