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Non‐degradative dissolution and acetylation of ball‐milled plant cell walls: high‐resolution solution‐state NMR
Author(s) -
Lu Fachuang,
Ralph John
Publication year - 2003
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2003.01817.x
Subject(s) - dissolution , lignin , chemistry , chloroform , cell wall , solvent , proton nmr , nuclear magnetic resonance spectroscopy , organic chemistry , chemical engineering , engineering , biochemistry
Summary Two solvent systems for fully dissolving, and optionally derivatizing, finely ground plant cell wall material at room temperature are described: dimethylsulfoxide (DMSO) and tetrabutylammonium fluoride (TBAF) or N ‐methylimidazole (NMI). In situ acetylation produces acetylated cell walls (Ac‐CWs) that are fully soluble in chloroform. Lignin structures tested remain fully intact. The dispersion of 13 C– 1 H correlations afforded by two‐dimensional (2D) nuclear magnetic resonance (NMR) experiments reveals the major lignin units, allowing the whole lignin fraction to be analyzed by high‐resolution solution‐state NMR methods for the first time. Non‐degradative cell wall dissolution offers the potential to analyze polysaccharide components, and improve current cell wall analytical methods by using standard homogeneous solution‐state chemistry.