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Activation tagging, a novel tool to dissect the functions of a gene family
Author(s) -
Nakazawa Miki,
Ichikawa Takanari,
Ishikawa Akie,
Kobayashi Hiroko,
Tsuhara Yumi,
Kawashima Mika,
Suzuki Kumiko,
Muto Shu,
Matsui Minami
Publication year - 2003
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2003.01758.x
Subject(s) - mutant , gene , genetics , biology , arabidopsis , allele , dominance (genetics) , gene family , dna , mutation , microbiology and biotechnology , gene expression
Summary In a screen for morphological mutants from the T 1 generation of approximately 50 000 activation‐tagging lines, we isolated four dominant mutants that showed hyponastic leaves, downward‐pointing flowers and decreased apical dominance. We designated them isoginchaku (iso) . The iso‐1D and iso‐2D are allelic mutants caused by activation of the AS2 gene. The T‐DNAs were inserted in the 3′ downstream region of AS2 . Iso‐3D and iso‐4D are the other allelic mutants caused by activation of the ASL1/LBD36 gene. These two genes belong to the AS2 family that is composed of 42 genes in Arabidopsis . The only recessive mutation isolated from this gene family was of AS2 , which resulted in a leaf morphology mutant. Applying reverse genetics using a database of activation‐tagged T‐DNA flanking sequences, we found a dominant mutant that we designated peacock1‐D (pck1‐D) in which the ASL5/LBD12 gene was activated by a T‐DNA. The pck1‐D mutants have lost apical dominance, have epinastic leaves and are sterile. These results strongly suggest that activation tagging is a powerful mutant‐mining tool especially for genes that make up a gene family.