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High‐Cot sequence analysis of the maize genome
Author(s) -
Yuan Yinan,
SanMiguel Phillip J.,
Bennetzen Jeffrey L.
Publication year - 2003
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2003.01716.x
Subject(s) - shotgun sequencing , shotgun , genome , dna sequencing , biology , genetics , hybrid genome assembly , computational biology , whole genome sequencing , gene , dna , sequence (biology)
Summary Higher eukaryotic genomes, including those from plants, contain large amounts of repetitive DNA that complicate genome analysis. We have developed a technique based on DNA renaturation which normalizes repetitive DNA, and thereby allows a more efficient outcome for full genome shotgun sequencing. The data indicate that sequencing the unrenatured outcome of a Cot experiment, otherwise known as High‐Cot DNA, enriches genic sequences by more than fourfold in maize, from 5% for a random library to more than 20% for a High‐Cot library. Using this approach, we predict that gene discovery would be greater than 95% and that the number of sequencing runs required to sequence the full gene space in maize would be at least fourfold lower than that required for full‐genome shotgun sequencing.