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Interaction between two cis ‐acting elements, ABRE and DRE, in ABA‐dependent expression of Arabidopsis rd29A gene in response to dehydration and high‐salinity stresses
Author(s) -
Narusaka Yoshihiro,
Nakashima Kazuo,
Shinwari Zabta K.,
Sakuma Yoh,
Furihata Takashi,
Abe Hiroshi,
Narusaka Mari,
Shinozaki Kazuo,
YamaguchiShinozaki Kazuko
Publication year - 2003
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2003.01708.x
Subject(s) - transactivation , arabidopsis , promoter , abscisic acid , reporter gene , gene expression , biology , microbiology and biotechnology , gene , electrophoretic mobility shift assay , chemistry , mutant , biochemistry
Summary Many abiotic stress‐inducible genes contain two cis ‐acting elements, namely a dehydration‐responsive element (DRE; TACCGACAT) and an ABA‐responsive element (ABRE; ACGTGG/TC), in their promoter regions. We precisely analyzed the 120 bp promoter region (−174 to −55) of the Arabidopsis rd29A gene whose expression is induced by dehydration, high‐salinity, low‐temperature, and abscisic acid (ABA) treatments and whose 120 bp promoter region contains the DRE, DRE/CRT‐core motif (A/GCCGAC), and ABRE sequences. Deletion and base substitution analyses of this region showed that the DRE‐core motif functions as DRE and that the DRE/DRE‐core motif could be a coupling element of ABRE. Gel mobility shift assays revealed that DRE‐binding proteins (DREB1s/CBFs and DREB2s) bind to both DRE and the DRE‐core motif and that ABRE‐binding proteins (AREBs/ABFs) bind to ABRE in the 120 bp promoter region. In addition, transactivation experiments using Arabidopsis leaf protoplasts showed that DREBs and AREBs cumulatively transactivate the expression of a GUS reporter gene fused to the 120 bp promoter region of rd29A . These results indicate that DRE and ABRE are interdependent in the ABA‐responsive expression of the rd29A gene in response to ABA in Arabidopsis .