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Arabidopsis microarrays identify conserved and differentially expressed genes involved in shoot growth and development from distantly related plant species
Author(s) -
Horvath David P.,
Schaffer Robert,
West Mark,
Wisman Ellen
Publication year - 2003
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2003.01706.x
Subject(s) - arabidopsis , biology , dna microarray , gene , genetics , arabidopsis thaliana , gene expression profiling , conserved sequence , gene expression , microarray analysis techniques , computational biology , peptide sequence , mutant
Summary Expressed sequence tags (EST)‐based microarrays are powerful tools for gene discovery and signal transduction studies in a small number of well‐characterized species. To explore the usefulness of this technique for poorly characterized species, we have hybridized the 11 522‐element Arabidopsis microarrays with labeled cDNAs from mature leaf and shoot apices from several different species. Expression of 23 to 47% of the genes on the array was detected, demonstrating that a large number of genes from distantly related species can be surveyed on Arabidopsis arrays. Differential expression of genes with known functions was indicative of the physiological state of the tissues tested. Genes involved in cell division, stress responses, and development were conserved and expressed preferentially in growing shoots.