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Enhancement of growth by expression of poplar cellulase in Arabidopsis thaliana
Author(s) -
Park Yong Woo,
Tominaga Rumi,
Sugiyama Junji,
Furuta Yuzo,
Tanimoto Eiichi,
Samejima Masahiro,
Sakai Fukumi,
Hayashi Takahisa
Publication year - 2003
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2003.01696.x
Subject(s) - cellulase , xyloglucan , cellulose , arabidopsis thaliana , cell wall , microfibril , arabidopsis , chemistry , botany , biophysics , biochemistry , biology , gene , mutant
Summary To study the role of cellulose and cellulase in plant growth, we expressed poplar cellulase ( PaPopCel1 ) constitutively in Arabidopsis thaliana . Expression increased the size of the rosettes due to increased cell size. The change in growth was accompanied by changes in biomechanical properties due to cell wall structure indicative of decrease in xyloglucan cross‐linked with cellulose microfibrils by chemical analysis and nuclear magnetic resonance (NMR) spectra. The result supports the concept that the paracrystalline sites of cellulose microfibrils are attacked by poplar cellulase to loosen xyloglucan intercalation and this irreversible wall modification promotes the enlargement of plant cells.