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Polyribosome loading of spinach mRNAs for photosystem I subunits is controlled by photosynthetic electron transport
Author(s) -
Sherameti Irena,
Nakamura Masayaki,
Yamamoto Yoshiharu Y.,
Pfannschmidt Thomas,
Obokata Junichi,
Oelmüller Ralf
Publication year - 2002
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2002.01452.x
Subject(s) - spinach , photosystem ii , photosynthesis , polysome , p680 , electron transport chain , dcmu , biology , photosystem i , cytoplasm , biophysics , chemistry , microbiology and biotechnology , biochemistry , gene , ribosome , rna
Summary In light‐, but not in dark‐grown spinach seedlings, the mRNAs for the nuclear‐encoded photosystem I subunits D, F and L are associated with polyribosomes and this association is prevented by the application of 3‐(3′,4′‐dichlorophenyl)‐1,1′‐dimethyl urea (DCMU), an inhibitor of the photosynthetic electron transport. To identify the cis ‐elements which are responsible for this regulation, we generated a series of chimeric PsaD constructs and tested them in transgenic tobacco. The spinach PsaD 5′‐untranslated region is sufficient to confer light‐ and photosynthesis‐dependent polyribosome association onto the uidA reporter gene, while the tobacco PsaD 5′‐untranslated region directs constitutive polyribosome association. These results are discussed with regard to signals from photosynthetic electron flow which control processes in the cytoplasm.