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Comprehensive transcript profiling of Pto‐ and Prf‐mediated host defense responses to infection by Pseudomonas syringae pv. tomato
Author(s) -
Mysore Kirankumar S.,
Crasta Oswald R.,
Tuori Robert P.,
Folkerts Otto,
Swirsky Peter B.,
Martin Gregory B.
Publication year - 2002
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2002.01424.x
Subject(s) - pseudomonas syringae , biology , gene , effector , complementary dna , genetics , plant disease resistance , gene expression profiling , gene expression , signal transduction , microbiology and biotechnology
Summary The disease resistance gene Pto encodes a serine/threonine protein kinase that confers resistance in tomato to Pseudomonas syringae pv. tomato strains that express the effector protein AvrPto. Pto‐mediated resistance to bacterial speck disease also requires Prf, a protein with leucine‐rich repeats and a putative nucleotide‐binding site, although the role of Prf in the defense pathway is not known. We used GeneCalling, an open‐architecture, mRNA‐profiling technology, to identify genes that are either induced or suppressed in leaves 4 h after bacterial infection in the Pto‐ and Prf‐mediated tomato‐ Pseudomonas ( avrPto ) interaction. Over 135 000 individual cDNA fragments representing an estimated 90% of the transcripts expressed in tomato leaves were examined and 432 differentially expressed genes were identified. The genes encode over 25 classes of proteins including 11 types of transcription factors and many signal transduction components. Differential expression of 91% of the genes required both Pto and Prf . Interestingly, differential expression of 32 genes did not require Pto but was dependent on Prf . Thus, our data support a role for Prf early in the Pto pathway and indicate that Prf can also function as an independent host recognition determinant of bacterial infection. Comprehensive expression profiling of the Pto ‐mediated defense response allows the development of many new hypotheses about the molecular basis of resistance to bacterial speck disease.