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Light regulated modulation of Z‐box containing promoters by photoreceptors and downstream regulatory components, COP1 and HY5, in Arabidopsis
Author(s) -
Yadav Vandana,
Kundu Snehangshu,
Chattopadhyay Debasis,
Negi Prem,
Wei Ning,
Deng XingWang,
Chattopadhyay Sudip
Publication year - 2002
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2002.01395.x
Subject(s) - promoter , photomorphogenesis , mutant , arabidopsis , biology , genetics , gene , dna , microbiology and biotechnology , gene expression
Summary The Z‐box is one of the light‐responsive elements (LREs) found in the promoters of light inducible genes. We have studied the light responsive characteristics of Z‐box containing synthetic as well as native promoters. We show that promoters with Z‐box as a single LRE or paired with another LRE can respond to a broad spectrum of light. The response is primarily mediated by phyA, phyB and CRY1 photoreceptors at their respective wavelengths of light. We have demonstrated that CAB1 and Z‐GATA containing promoters are down‐regulated in hy5 mutants in the light. On the other hand, a promoter with Z‐box alone is down‐regulated in hy5 mutants both in dark and in light conditions, suggesting involvement of a similar regulatory system in the regulation of the promoter in two distinct developmental pathways: skotomorphogenesis and photomorphogenesis. Furthermore, similar to the CAB1 promoter, a Z‐GATA containing promoter is derepressed in cop1 mutants in the dark. DNA–protein interaction studies reveal the presence of a DNA‐binding activity that is specific to Z‐box. These results provide insights into the regulation of the Z‐box LRE mediated by various light signaling components.