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The broad‐spectrum potato cyst nematode resistance gene ( Hero ) from tomato is the only member of a large gene family of NBS‐LRR genes with an unusual amino acid repeat in the LRR region
Author(s) -
Ernst Karin,
Kumar Amar,
Kriseleit Doris,
Kloos DorotheeU.,
Phillips Mark S.,
Ganal Martin W.
Publication year - 2002
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2002.01341.x
Subject(s) - biology , gene , genetics , r gene , potato cyst nematode , globodera rostochiensis , cosmid , gene family , plant disease resistance , microbiology and biotechnology , genome , nematode , ecology
Summary The Hero gene of tomato is a broad spectrum resistance gene that confers a high level of resistance to all pathotypes of the potato cyst nematodes Globodera rostochiensis and partial resistance to G. pallida . The gene was identified by map‐based cloning, sequencing and complementation analysis of two susceptible tomato lines with an array of 13 overlapping cosmids spanning a total distance of 135 kb. Hero encodes a protein with a nucleotide‐binding site (NBS) and a leucine‐rich‐repeat (LRR) domain and is a member of a gene family of 14 highly homologous genes, which are clustered within a continuous 118‐kb region. The isolated Hero gene displayed resistance to various G. rostochiensis pathotypes and partial resistance to G. pallida pathotype Pa 2/3 in transgenic tomato lines. None of the Hero homologues conferred resistance to G. rostochiensis pathotypes. Hero can be distinguished from its homologues by the length of a compound hexanucleotide microsatellite, which codes for a charged and repetitive amino acid domain within the LRR. We propose that the expansion of this microsatellite may be involved in the evolution of the Hero resistance gene.