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At‐HSP17.6A , encoding a small heat‐shock protein in Arabidopsis , can enhance osmotolerance upon overexpression
Author(s) -
Sun Weining,
Bernard Catherine,
Van De Cotte Brigitte,
Van Montagu Marc,
Verbruggen Nathalie
Publication year - 2001
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2001.01107.x
Subject(s) - arabidopsis , heat shock protein , biology , arabidopsis thaliana , osmotic shock , cytosol , hspa14 , gene , hspa12a , chaperone (clinical) , biochemistry , hsp70 , microbiology and biotechnology , enzyme , mutant , medicine , pathology
Summary Owing to their sessile lifestyle, it is crucial for plants to acquire stress tolerance. The function of heat‐shock proteins, including small heat‐shock proteins (smHSPs), in stress tolerance is not fully explored. To gain further knowledge about the smHSPs, the gene that encoded the cytosolic class II smHSP in Arabidopsis thaliana ( At‐HSP17.6A ) was characterized. The At‐HSP17.6A expression was induced by heat and osmotic stress, as well as during seed development. Accumulation of At‐HSP17.6A proteins could be detected with heat and at a late stage of seed development, but not with osmotic stress, suggesting stress‐induced post‐transcriptional regulation of At‐HSP17.6A expression. Overproduction of At‐HSP17.6A could increase salt and drought tolerance in Arabidopsis . The chaperone activity of At‐HSP17.6A was demonstrated in vitro .