Premium
The auxin signal for protoplast swelling is perceived by extracellular ABP1
Author(s) -
Steffens Bianka,
Feckler Christian,
Palme Klaus,
Christian May,
Böttger Michael,
Lüthen Hartwig
Publication year - 2001
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2001.01103.x
Subject(s) - auxin , coleoptile , protoplast , arabidopsis , arabidopsis thaliana , hypocotyl , heterologous , extracellular , biology , microbiology and biotechnology , epitope , antibody , biochemistry , chemistry , botany , gene , immunology , mutant
Summary Protoplasts of corn coleoptiles and Arabidopsis hypocotyls respond to the plant hormone auxin with a rapid change in volume. We checked the effect of antibodies directed against epitopes of auxin‐binding protein 1 from Arabidopsis thaliana (AtERabp1) and Zea mays (ZmERabp1), respectively. Antibodies raised against the C‐terminus of AtERabp1 inhibited the response to auxin, while antibodies raised against a part of box a, the putative auxin‐binding domain, induced a swelling response similar to that caused by auxin treatment. Synthetic C‐terminal oligopeptides of ZmERabp1 also caused a swelling response. These effects occurred regardless of whether the experiments were carried out with homologous (anti‐AtERabp1 antibodies on Arabidopsis protoplasts or anti‐ZmERabp1 antibodies in maize protoplasts) or heterologous immunological tools. The results indicate that the auxin signal for protoplast swelling is perceived by extracellular ABP1.