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The protein kinases AtMAP3Kε1 and BnMAP3Kε1 are functional homologues of S. pombe cdc7p and may be involved in cell division
Author(s) -
Jouannic Stefan,
Champion Anthony,
SeguiSimarro JoséMaria,
Salimova Ekaterina,
Picaud Alain,
Tregear James,
Testillano Pilar,
Risueño MariaCarmen,
Simanis Viesturs,
Kreis Martin,
Henry Yves
Publication year - 2001
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2001.01065.x
Subject(s) - schizosaccharomyces pombe , biology , arabidopsis thaliana , cytokinesis , gene , kinase , cyclin dependent kinase 1 , arabidopsis , polo like kinase , cyclin dependent kinase 7 , saccharomyces cerevisiae , genetics , schizosaccharomyces , microbiology and biotechnology , cell division , cell cycle , mutant , cell , cyclin dependent kinase 2
Summary We identified an Arabidopsis thaliana gene, AtMAP3Kε1, and a Brassica napus cDNA, BnMAP3Kε1 , encoding functional protein serine/threonine kinases closely related to cdc7p and Cdc15p from Schizosaccharomyces pombe and Saccharomyces cerevisiae , respectively. This is the first report of cdc7‐related genes in non‐fungal eukaryotes; no such genes have as yet been identified in Metazoans. The B. napus protein is able to partially complement a cdc7 loss of function mutation in S. pombe . RT–PCR and in situ hybridisation revealed that the A. thaliana and B. napus genes are expressed in both the sporophytic and the gametophytic tissues of the respective plant species and revealed further that expression is highest in dividing cells. Moreover, AtMAP3Kε1 gene expression is cell cycle‐regulated, with higher expression in G2‐M phases. Our results strongly suggest that the plant cdc7p‐related protein kinases are involved in a signal transduction pathway similar to the SIN pathway, which positively regulates cytokinesis in S. pombe .