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Analysis of the SINE S1 Pol III promoter from Brassica; impact of methylation and influence of external sequences
Author(s) -
Arnaud Philippe,
Yukawa Yasushi,
Lavie Laurence,
Pélissier Thierry,
Sugiura Masahiro,
Deragon JeanMarc
Publication year - 2001
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2001.01029.x
Subject(s) - transcription (linguistics) , transposable element , biology , genetics , methylation , promoter , locus (genetics) , dna methylation , general transcription factor , response element , sine , gene , mutant , gene expression , mathematics , philosophy , linguistics , geometry
Summary Transcription is an important control point in the transposable element mobilization process. To better understand the regulation of the plant SINE (Short Interspersed Elements) S1, its promoter sequence was studied using an in vitro pol III transcription system derived from tobacco cells. We show that the internal S1 promoter can be functional although upstream external sequences were found to enhance this basal level of transcription. For one putative ‘master’ locus (na7), three CAA triplets (in positions –12, –7 and –2) and two overlapping TATA motifs (in positions –54 to –43) were important to stimulate transcription. For this locus, two transcription initiation regions were characterized, one centered on position + 1 (first nucleotide of the S1 element) and one centered on position − 19 independently of the internal motifs. The CAA triplets only influence transcription in + 1 and work in association with the internal motifs. We show that methylation can inhibit transcription at the na7 locus. We also observe that S1 RNA is cleaved in a smaller Poly (A) minus product by a process analogous to the maturation of mammalian SINEs.