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A highly specific pathogen‐responsive promoter element from the immediate‐early activated CMPG1 gene in Petroselinum crispum
Author(s) -
Kirsch Christoph,
Logemann Elke,
Lippok Bernadette,
Schmelzer Elmon,
Hahlbrock Klaus
Publication year - 2001
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2001.01015.x
Subject(s) - gene , biology , elicitor , reporter gene , genetics , transcription factor , transgene , gene expression , regulation of gene expression , arabidopsis , arabidopsis thaliana , transcription (linguistics) , genetically modified crops , promoter , microbiology and biotechnology , mutant , linguistics , philosophy
Summary Within the complex signalling network from pathogen‐derived elicitor perception to defense‐related gene activation, some immediate‐early responding genes may have pivotal roles in downstream transcriptional regulation. We have identified the parsley ( Petroselinum crispum ) ELI17 gene as a particularly fast‐responding gene possessing a new type of W box‐containing, elicitor‐responsive promoter element, E17. Highly selective E17‐mediated reporter gene expression at pathogen infection sites in transgenic Arabidopsis thaliana plants demonstrated the potential of this promoter element for designing new strategies in resistance breeding as well as for further analysis of the early components of defense‐related gene activation mechanisms. The protein encoded by the ELI17 gene exhibits various structural characteristics of established transcription factors and is designated as a CMPG protein according to the first four strictly conserved amino acids defining a newly emerging class of plant‐specific proteins.