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Analysis of the function of two circadian‐regulated CONSTANS‐LIKE genes
Author(s) -
Ledger Susan,
Strayer Carl,
Ashton Fern,
Kay Steve A.,
Putterill Jo
Publication year - 2001
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2001.01003.x
Subject(s) - arabidopsis , circadian rhythm , biology , transgene , gene , circadian clock , genetics , phenotype , zinc finger , gene expression , arabidopsis thaliana , microbiology and biotechnology , mutant , transcription factor , neuroscience
Summary The Arabidopsis genes CONSTANS‐LIKE 1 ( COL1 ) and CONSTANS‐LIKE 2 ( COL2 ) are predicted to encode zinc finger proteins with ∼67% amino acid identity to the protein encoded by the flowering‐time gene CONSTANS (CO). We show that the circadian clock regulates expression of COL1 and COL2 with a peak in transcript levels around dawn. We analyzed transgenic plants misexpressing COL1 , COL2 and CO . Unlike CO , altered expression of COL1 and COL2 in transgenic plants had little effect on flowering time. However, analysis of circadian phenotypes in the transgenic plants showed that over‐expression of COL1 can shorten the period of two distinct circadian rhythms. Experiments with the highest COL1 over‐expressing line indicate that its circadian defects are fluence rate‐dependent, suggesting an effect on a light input pathway(s).

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