Premium
Identification of an Arabidopsis thaliana mutant accumulating threonine resulting from mutation in a new dihydrodipicolinate synthase gene
Author(s) -
Sarrobert Catherine,
Thibaud MarieChristine,
ContardDavid Pascale,
Gineste Sandrine,
Bechtold Nicole,
Robaglia Christophe,
Nussaume Laurent
Publication year - 2000
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2000.00884.x
Subject(s) - mutant , arabidopsis thaliana , arabidopsis , biology , gene , threonine , mutation , amino acid , biochemistry , wild type , genetics , microbiology and biotechnology , serine , enzyme
Summary A novel Arabidopsis DHDPS gene named DHDPS2 was found through identification of a mutant by promoter trapping. The mutation promotes a reduction of growth resulting from combination of a defect in lysine biosynthesis and accumulation of a toxic level of threonine or derived products. The mutant also modifies the amino acid composition issuing from the pyruvate and aspartate pathways, affecting mainly the root compartment. These data are in accordance with the expression of DHDPS2 in the root apex as visualized by expression of the GUS reporter gene. This suggests that a large proportion of the amino acids derived from pyruvate and aspartate are synthesized in this organ.