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Photocontrol of subcellular partitioning of phytochrome‐B:GFP fusion protein in tobacco seedlings
Author(s) -
Gil Patricia,
Kircher Stefan,
Adam Eva,
Bury Erik,
KozmaBognar Laszlo,
Schäfer Eberhard,
Nagy Ferenc
Publication year - 2000
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2000.00730.x
Subject(s) - cryptochrome , phytochrome , photomorphogenesis , visual phototransduction , phototropin , biophysics , blue light , biology , nuclear transport , microbiology and biotechnology , subcellular localization , light cycle , phytochrome a , arabidopsis , red light , botany , nucleus , biochemistry , cell nucleus , gene , circadian clock , optics , cytoplasm , physics , circadian rhythm , neuroscience , retinal , mutant
Summary Photomorphogenesis of higher plants is regulated by photoreceptors including the red/far‐red light‐absorbing phytochromes, blue‐UV/A sensing cryptochromes and as yet uncharacterized UV/B receptors. Specific phototransduction pathways that are controlled by either individual or interacting photoreceptors mediate regulation. Phytochrome B (phyB) is the major red light‐sensing photoreceptor. Phototransduction mediated by this light sensor has been shown to include light‐dependent nuclear import and interaction of phyB with transcription factor‐like proteins in the nucleus. Here we report that nuclear import of phyB and physiological responses regulated by this photoreceptor exhibit very similar wavelength‐ and fluence rate‐dependence. Nuclear import of phyB is insensitive to single red, blue and far‐red light pulses. It is induced by continuous red light and to a lesser extent by continuous blue light, whereas far‐red light is completely ineffective. The data presented indicate that light‐dependent partitioning of phyB exhibits features characteristic of blue light responsiveness amplification, a phenomenon that is thought to be mediated by interaction of phyB with CRY1.