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Disruption of an Arabidopsis cytoplasmic ribosomal protein S13‐homologous gene by transposon‐mediated mutagenesis causes aberrant growth and development
Author(s) -
Ito Takuya,
Kim GyungTae,
Shinozaki Kazuo
Publication year - 2000
Publication title -
the plant journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.058
H-Index - 269
eISSN - 1365-313X
pISSN - 0960-7412
DOI - 10.1046/j.1365-313x.2000.00728.x
Subject(s) - arabidopsis , gene , biology , homologous chromosome , mutagenesis , transposable element , transposon mutagenesis , genetics , ribosomal rna , ribosomal protein , homologous recombination , insertional mutagenesis , mutant , ribosome , rna
Summary We identified a Dissociation ( Ds ) transposon‐inserted Arabidopsis mutant of a gene ( AtRPS13A ) homologous to cytoplasmic ribosomal protein (RP) S13. We named our mutant pointed first leaf ( pfl ) 2 because of its similar phenotype to the pfl1 mutant of the RPS18 gene. This mutant caused multiple phenotypic changes, including aberrant leaf and trichome morphology, retarded root growth, and late flowering. Microscopic analysis showed that the first leaf blade of pfl2 contained a significantly reduced number of palisade cells, which suggests that the mutant phenotype was caused by reduced cell division. However, no phenotypic changes were observed during reproductive growth. In Arabidopsis , the RPS13 protein was encoded by a small expressed gene family including AtRPS13A . A pfl1 pfl2 double mutant showed no additive effect. These results suggest that RPS13 functions in quantitative and pleiotropic ways during growth and development, and that mutations at different kinds of RP gene loci are accumulatable without serious growth defects because they belong to small gene families.